Journal: Cell Proliferation

Article Title: Cell volume and rate of proliferation, but not protein expression pattern, distinguish pup/intimal smooth muscle cells from subcultured adult smooth muscle cells

doi: 10.1046/j.0960-7722.2001.00212.x

Figure Lengend Snippet: Cumulative population doubling times and estimated median cell volumes for subcultured medial SMCs and cobblestone SMCs. The population doubling time and the median forward scatter pulse height were determined as described in Materials and Methods for three cultures of subcultured medial SMCs at passages 7, 9 and 11 after primary culture, and for three cobblestone morphology SMC clones at the second passage after re‐establishing the culture from frozen cell stocks ( n ) and at each alternate passage thereafter

Article Snippet: Cobblestone morphology clonal cell lines were established by dilute plating as described previously ( Grainger 1992 ; Kemp et al. 1995 ).

Techniques: Clone Assay, Cell Culture